為建立快速定量檢測(cè)家禽樣本中禽白血病病毒抗原的方法，對(duì)禽白血病病毒p27蛋白單克隆抗體細(xì)胞株2E5和3D5進(jìn)行復(fù)蘇、鑒定，通過(guò)反應(yīng)條件優(yōu)化，建立了禽白血病病毒抗原高敏熒光微球快速檢測(cè)方法。該方法能夠快速定量檢測(cè)家禽樣本中禽白血病病毒，敏感性高、特異性強(qiáng)，對(duì)其他家禽病原無(wú)交叉反應(yīng)且具有良好的檢測(cè)重復(fù)性。對(duì)采自全國(guó)的240份臨床家禽樣品進(jìn)行檢測(cè)，同時(shí)用美國(guó)IDEXX公司生產(chǎn)的ELISA抗原試劑盒開(kāi)展比較，結(jié)果發(fā)現(xiàn)兩種方法符合率達(dá)93.98%，其中陽(yáng)性樣品符合率為90.83%，陰性樣品符合率為95.83%；組內(nèi)與組間變異系數(shù)分別低于10%和15%。本研究建立的禽白血病病毒p27抗原高敏熒光微球快速檢測(cè)方法，特異性高、敏感性強(qiáng)，操作簡(jiǎn)單、快速，具有較高的應(yīng)用推廣價(jià)值。
Establishment of the Highly Sensitive and Rapid Fluorescence Quantitative Detection for Avian Leukemia Virus
In order to establish a rapid and quantitative method for detection of avian leukosis virus（ALV）in poultry samples，2E5 and 3D5，the monoclonal antibody cell strains of ALV p27 protein were revived and identified，a highly sensitive and rapid fluorescence quantitative detection method，with high sensitivity and specificity and good test repeatability and no cross reaction to other pathogens，was established through optimization of reaction conditions，by which，ALV could be rapidly and quantitatively detected from poultry samples. 240 clinical samples collected across China were detected and compared with ELISA kit produced by IDEXX. It was found that the coincidence rate of the two methods was 93.98%，in which the coincidence rate of positive samples was 90.83%，and that of negative ones was 95.83%. The coefficients of variation in intra group and inter group were lower than 10% and 15%，respectively. In conclusion，the established method was worthy of being applied and expanded as supported by its high specificity and sensitivity，easy and rapid operation.
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