沙門氏菌、單核細胞增生李斯特菌和金黃色葡萄球菌是食品中常見的致病菌，在國內(nèi)外常引起食源性疾病。為解決食源性疾病中的微生物檢測問題，本研究將免疫磁珠技術(shù)和ATP發(fā)光技術(shù)聯(lián)合用于食品微生物檢測，選擇沙門氏菌、單核細胞增生李斯特菌病和金黃色葡萄球菌為檢測對象，建立快速檢測這3種常見食源性病原菌的富集及檢測新方法。該方法在顯著縮短預(yù)增菌時間的條件下，通過免疫磁珠的富集作用獲得與常規(guī)增菌時間同樣的效果，樣品中的微生物濃度增加了10倍，大大提高了樣本檢出率，縮短了檢測周期。結(jié)果表明，本研究建立的免疫磁珠富集后聯(lián)合ATP發(fā)光技術(shù)具有快速、敏感、特異和低廉的優(yōu)點，可用于檢測食品中沙門氏菌、單核細胞增生李斯特菌以及金黃色葡萄球菌，具有較好的推廣應(yīng)用前景。
Development of a Technology of Immunomagnetic BeadsCombined with ATP
Chemiluminescence for Detection of three Kinds of FoodborneBacteria
At present，Salmonella，Listeria monocytogenes and Staphylococcus aureus arecommon pathogenic bacteria in food，usually causing foodborne diseases. In order to solvethe problems of microbial detection，the immunomagnetic beads technology and ATPluminescence technology were combined，and a new method was established for rapid detectionof the three foodborne pathogens. Results showed that，under the condition of significantlyshortening the pre-enrichment time of bacteria，the same effects as the conventional enrichment timewas obtained by the immunomagnetic beads，and the microbial concentration of the samples wasincreased by 10 times，which greatly improved the sample detection rate and shortened thedetection cycle. In conclusion，the established method in this study was rapid，sensitive，specific and low-cost in detection of Salmonella，Listeria monocytogenes and Staphylococcusaureus in food，and it would have application prospects.
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