為了解云南邊境地區(qū)近年來阿卡斑病毒（Akabanne disease virus，AKAV）流行情況，2017—2018年連續(xù)2年在云南省與緬甸、老撾、越南接壤的12個邊境縣采集牛血清，應用cELISA方法進行AKAV抗體檢測。結果顯示：2017—2018年共檢測牛血清樣品1860份，檢出陽性樣品108份，陽性率為5.8%，顯示這些地區(qū)存在一定的AKAV感染；各縣AKAV抗體陽性率在0~24.4%之間，差異較大，其中抗體陽性率最高的為富寧縣，2017年和2018年的陽性率分別為24.4%和20.0%，而瑞麗連續(xù)2年未檢出抗體陽性；黃牛AKAV抗體陽性率（6.6%）高于水牛（4.7%），差異顯著（P<0.05）；入境?？贵w陽性率（6.5%）高于境內牛（3.0%），差異顯著（P<0.05）；養(yǎng)殖場（9.0%）、交易市場（8.4%）、屠宰場（7.6%）的牛AKAV抗體陽性率高于村寨散養(yǎng)戶（2.5%），差異極顯著（P<0.01）。結果表明，AKAV在云南邊境地區(qū)存在一定的流行，其流行趨勢受入境動物影響。結果提示，應繼續(xù)加強云南省邊境地區(qū)AKAV等蟲媒病毒病的監(jiān)控，及早發(fā)現和控制該病流行。
AntibodyDetection of Akabane Disease Virus in Border Areas
ofYunnan Province from 2017 to 2018
Inorder to investigate the prevalence of Akabanne disease virus（AKAV）in Yunnan border areasin recent years，bovineserum samples were continuously collected in 12 counties bordering on Myanmar，Laos and Vietnam duringthe period from 2017 to 2018，and the specific antibodies were tested by cELISA.Results showed that 1 860 serum samples were tested，involving 108 positivesamples with the positive rate of 5.8%，indicating that there was certain AKAV infectionin these areas；thepositive rate of AKAV antibodies in each county ranged from 0 to 24.4%，and the rate in FuningCounty was the highest，which was 24.4% in 2017 and 20.0% in 2018. No anypositive antibodies were detected in Ruili City for two consecutive years；the positive rate ofAKAV antibody in yellow cattle was 6.6%，which was higher than that in buffalo（4.7%）with significantdifference（P<0.05）；thepositive rate in imported cattle（6.5%）was significantly higher than that in domesticcattle（3.0%）with significantdifference（P<0.05）；andthe rate in cattle in farms（9.0%），trading markets（8.4%） and slaughterhouses（7.6%）was higher than that in free-range households invillages（2.5%） with significantdifference（P<0.01）. The results showedthat AKAV was prevalent to some extent in Yunnan border areas，which was influenced bythe imported animals. Therefore，it was suggested to strengthen the surveillancefor AKAV and other arbovirus diseases in Yunnan border areas，so as to detect andcontrol such diseases at the early stage.
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