20世紀(jì)90年代，本實驗室從云南省哨兵動物采集的血液中分離到7種血清型藍(lán)舌病病毒（bluetongue virus，BTV）。但這些毒株的遺傳特征至今仍不清楚，因而阻礙了我國的BTV演化歷史分析。為掌握云南省早期流行BTV毒株的遺傳特征，對1995—1997年云南省分離的25株BTV毒株的基因組節(jié)段2、3、7和10（Seg-2、-3、-7、-10）進(jìn)行一步法RT-PCR擴(kuò)增、測序以及序列分析。Seg-2序列分析顯示，1995—1997年云南省分離到的7種不同血清型（BTV-1、-2、-3、-4、-12、-15、-16）BTV毒株，分屬A、B、G、H、I和J等6種基因型；BTV-12型毒株的Seg-2為西方地域型，其余毒株的Seg-2則屬于東方地域型；Seg-3和Seg-10序列分析顯示，25株BTV毒株均為東方地域型；Seg-7序列分析顯示，1株BTV-2型、2株BTV-12型和1株BTV-16型毒株屬于西方地域亞型，并與南非和荷蘭BTV毒株的Seg-7具有最近的親緣關(guān)系，其余毒株則均為東方地域型。上述結(jié)果表明，云南省存在多種血清型BTV的流行，而Seg-2和Seg-7基因重配毒株的發(fā)現(xiàn)，提示國外的西方地域型毒株已侵入云南省，并在傳播過程中與我國毒株發(fā)生了基因重配。本研究為我國BTV的演化分析與溯源研究提供了數(shù)據(jù)參考。
Genetic Characteristics of“Historical Strains”of Bluetongue Virus
Isolated from Yunnan Province
In the 1990s，7 serotypes of bluetongue virus（BTV）were isolated from blood samples collected from sentinel animals in Yunnan Province by our laboratory. However，the genetic characteristics of these strains were still not clear，which hindered the study on the evolutionary history of BTV in China. In order to make clear the genetic characteristics of BTV strains spread early in Yunnan，one-step RT-PCR amplification，sequencing and sequence analysis were carried out for gene segments 2，3，7 and 10（Seg-2，-3，-7 and-10）of 25 BTV strains isolated in the province from 1995 to 1997. It was found that，by Seg-2 sequence analysis，Seven different BTV serotype strains（BTV-1，-2，-3，-4，-12，-15 and -16）were isolated，which fell into 6 nucleotypes of A，B，G，H，I and J，respectively；the Seg-2 of BTV-12 fell into Western topotype，but other strains belonged to Eastern topotype；it was showed that，by Seg-3 and Seg-10 sequence analysis，25 strains of BTV belonged to Eastern topotype；and by Seg-7 sequence analysis，one strain of BTV-2，two strains of BTV-12 and one strain of BTV-16 were clustered into Western topotype，and shared the closest genetic relationship with Seg-7 isolated from South Africa and Netherlands，and the other strains belonged to Eastern topotype. It was concluded that multiple serotypes of BTV were prevalent in Yunnan Province，and foreign Western topotype strains had been introduced into the province and reassorted with Chinese strains in the process of transmission as Seg-2 and Seg-7 gene reassortative isolates were detected. Some data was provided for evolution analysis and traceability study on BTV in China.
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