為檢測從內(nèi)蒙古發(fā)病牛場分離到的3株牛支原體（HS2019、HSZ2019、HSS2019）的致病性，對其進行本動物回歸試驗，通過觀察攻毒后的臨床癥狀、病理變化，以及應(yīng)用實時熒光定量PCR確定組織器官中支原體載量，分析3株支原體的毒力。結(jié)果顯示，3株牛支原體回歸牛體后均使試驗牛出現(xiàn)體溫升高、咳嗽、呼吸困難等臨床癥狀，解剖可觀察到試驗牛肺部損傷以及肺臟與胸腔粘連的病理變化，其中HS2019株較其他兩株引起的癥狀與病變更為明顯，組織臟器中的載菌量最高。結(jié)果表明，這3株支原體均有致病性，其中HS2019株致病性最強，可作為今后疫苗研制的預(yù)備菌株。本研究既為國內(nèi)疫苗的研制提供了菌株資源，也為今后牛支原體的免疫攻毒試驗提供了評價標準。
Comparison of the Virulence of Three Strains of Mycoplasma bovis
In order to test the virulence of three strains of Mycoplasma bovis（HS2019，HSZ2019 and HSS2019）isolated from an infected farm in Inner Mongolia，an animal regression test was carried out，the number of Mycoplasma in tissue and organs was determined by observing clinical symptoms and pathological changes after virus challenge as well as by real-time fluorescent quantitative PCR，then the virulence of the three strains was analyzed. The results showed that such clinical symptoms as increasing body temperature，cough and dyspnea appeared in experimental cattle after the regression test of the three strains. The lung lesions as well as adhesion between lung and thoracic cavity and other pathological changes could be observed after anatomy，especially HS2019 strain，by which more obvious symptoms and lesions were caused，and the volume of Mycoplasma in tissues was maximum. In conclusion，the three strains of Mycoplasma were all pathogenic，especially HS2019 strain whose virulence was the strongest，which could be used as the preparation strain for vaccine development in the future. By the study，strain resources were provided for the development of vaccines，and evaluation criterion for immune challenge test of Mycoplasma bovis in the future was also provided.
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