為了解湖南豬群豬圓環(huán)病毒1型（PCV1）的遺傳變異情況，以PCV1陽性DNA為模板，使用PCR方法分兩段擴(kuò)增PCV1全基因序列并進(jìn)行拼接。結(jié)果共獲得5個PCV1全基因組，其中3株全基因大小為1759 bp，另外2株分別為1758 bp和1760 bp。5株全基因組同源性為98.4%~99.7%，與國內(nèi)外的PCV1全基因組同源性為98.1%~99.7%；ORF2序列同源性為97.6%~99.6%，與國內(nèi)外的PCV1 ORF2同源性為96.1%~99.9%。系統(tǒng)發(fā)育樹顯示，5株P(guān)CV1均屬于同一分支，并顯示出一定的地理差異，但差異較小。結(jié)果表明，湖南省流行的PCV1毒株基因較為穩(wěn)定，分離株間差異較小。本研究為湖南省豬圓環(huán)病毒病防控及相關(guān)研究奠定了基礎(chǔ)。
Whole Genome Sequencing of Five Strains of PCV1 in Hunan Province and Analysis on Their Genetic Evolution
In order to identify the genetic variation of porcine circovirus type 1（PCV1）in pigs in Hunan Province，taking PCV1 positive DNA as a template，two sections of PCV1 gene sequence were amplified by PCR and then spliced together to form the whole genome. Five strains of whole genomes were obtained，in which，three were 1759 bp，and two were 1758 bp and 1760 bp，respectively. The homology of the whole genome was 98.4% to 99.7%，which shared the homology of 98.1% to 99.7% with other whole genomes of PCV1；the homology of ORF2 sequence was 97.6% to 99.6%，which shared the homology of 96.1% to 99.9% with other PCV1 ORF2. As indicated by the phylogenetic tree，all the five strains belonged to the same branch with slightly geographical difference. In conclusion，the strains were steadily prevalent in Hunan Province with little difference. A foundation was thereby provided for future prevention and control of PCV in Hunan and for relevant studies.
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